CYCLOPAMINE; V. CALIFORNICUM

Code: 239803-1MG D2-231

Biochem/physiol Actions

Product does not compete with ATP.

Primary TargetHh signaling in Shh-light2 assay

Cell permeable: yes

General description

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Your Price
€216.36 1MG
€266.12 inc. VAT

Biochem/physiol Actions

Product does not compete with ATP.

Primary TargetHh signaling in Shh-light2 assay

Cell permeable: yes

General description

Cyclopamine, V. californicum, CAS 4449-51-8, is a cell-permeable steroidal alkaloid & cholesterol mimic that specifically antagonizes Sonic Hedgehog signaling through direct interaction with Smo.

A cell-permeable steroidal alkaloid and cholesterol mimic that displays both teratogenic and antitumor activities. It disrupts cholesterol bio-synthesis and specifically antagonizes shh (Sonic Hedgehog) signaling pathway through direct interaction with Smo (smoothened), a distant relative of G-protein-coupled receptors. A valuable tool for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control. Also available as a 10 mM solution in Ethanol (Cat. No. 239806).

A cell-permeable, steroidal alkaloid and cholesterol mimic that displays both teratogenic and anti-tumor properties. Disrupts cholesterol biosynthesis and specifically antagonizes shh (Sonic Hedgehog) signaling through direct interaction with Smo (smoothened), a protein that is related to G-protein-coupled receptors. Useful for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Berman, D.M., et al. 2003. Nature,425, 846.Thayer, S.P., et al. 2003. Nature,425, 851.Watkins, D.N., et al. 2003. Nature422, 313.Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.Berman, D.M., et al. 2002. Science297, 1559.Taipale, J., et al. 2000. Nature406, 1005.Kim, S.K., and Melton, D.A. 1998. Proc. Natl. Acad. Sci. USA95, 13036.Cooper, M.K., et al. 1998. Science280, 1603.Incardona, J.P., et al. 1998. Development125, 3553.

Packaging

1, 5 mg in Glass bottle

Preparation Note

Ethanol stock solutions may require warming to achieve complete solubilization.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Carcinogenic / Teratogenic (D)

assay≥97% (HPLC)
coloroff-white
formsolid
InChI keyQASFUMOKHFSJGL-LAFRSMQTSA-N
InChI1S/C27H41NO2/c1-15-11-24-25(28-14-15)17(3)27(30-24)10-8-20-21-6-5-18-12-19(29)7-9-26(18,4)23(21)13-22(20)16(27)2/h5,15,17,19-21,23-25,28-29H,6-14H2,1-4H3/t15-,17+,19-,20-,21-,23-,24+,25-,26-,27-/m0/s1
manufacturer/tradenameCalbiochem®
potency20 nM IC50
Quality Level100
shipped inwet ice
solubilityethanol: 5 mg/mL, DMF: 10 mg/mL, DMSO: 4 mg/mL
storage conditionprotect from light, OK to freeze
storage temp.−20°C
Cas Number4449-51-8
This product has met the following criteria to qualify for the following awards:



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